C-Peptide-CHECK-1 Quantitative determination of C-Peptide in whole blood, plasma or serum samples

Human C-Peptide, a 31 aminoacid residue peptide having a molecular mass of approximately 3 000Da. (1) connects Insulin’s A and B chains within the proinsulin molecule (2, 3). Proinsulin is subsequently cleaved enzymatically into the beta-cells of the pancreas leading to an equimolar release of insulin and C-Peptide into the blood stream (4). The degradation rate of C.Peptide in the body being slower than that of insulin (half life of 20-30 minutes, compared with the half-life of insulin of just 3-5 minutes), its measurement is a more stable indicator of insulin secretion and beta-cell function (5). Due to its relative metabolic inertness, the C-Peptide levels in peripheral venous blood are about 5-6 times greater than insulin levels. In addition, another advantage of C-Peptide assay is its ability to distinguish endogenous from injected insulin. Therefore, a very low C-Peptide concentration confirms Type 1 diabetes and insulin dependence while elevated C-Peptide levels are associated to Type 2 diabetes or to the increased B-cell activity observed in insulinomas (6, 7, 8). The C-Peptide-CHECK-1 test is a rapid quantitative assay for the detection of C-Peptide in serum, plasma or whole blood. The method employs a unique combination of monoclonal- gold conjugate and monoclonal solid phase antibodies to identify C-Peptide in the test samples with a high degree of specificity. As the test sample flows through the absorbent device, the labelled antibody-gold conjugate binds to the C-Peptide forming an antibody-antigen complex. This complex binds to the anti- C-Peptide antibody in the positive reaction zone (T) and produces a pink-rose colour band. In the absence of C-Peptide, there is no line in the positive reaction zone (T). The reaction mixture continues flowing through the absorbent device past the reaction zone (T) and control zone (C). Depending on the C-Peptide concentration level, different lines of different intensities will appear on the reading window allowing the quantitative measurement of C-Peptide when used in combination with the VEDALAB’s readers EASYREADER® or EASYREADER+®.

Each kit contains

1. C-Peptide-CHECK-1reaction devices: 10 20
2. Disposable plastic pipettes: 10 20
3. Diluent in a dropper bottle: 2.5mL 5mL
4. Instruction leaflet: 1 1