HS-CRP-CHECK-1 High sensitive (Laboratories) Quantitative determination of C-Reactive Protein in whole blood, plasma or serum samples

C-reactive protein (CRP) is a non-specific, acute-phase reactant used to diagnose bacterial infectious disease and inflammatory disorders, such as acute rheumatic fever and rheumatoid arthritis (1). Published studies (2,3) suggest that cardiovascular disease (CVD) and peripheral vascular disease (PVD) are inflammatory processes due to myocardial necrosis and can result in low levels of inflammatory markers such as C-reactive protein. Therefore the determination of CRP level can be helpful to predict a healthy person’s risk of cardiovascular disease, heart attacks and strokes. The level of CRP correlates with peak levels of the MB isoenzyme of creatine kinase, but CRP peaks occur 1 to 3 days later. Failure of CRP to normalise may indicate ongoing damage to the heart tissue. Levels are not elevated in patients with angina. The measurement of CRP does not indicate where is occurring the inflammation which may come from cells in the fatty deposits in arterial walls reflecting the process of atherosclerosis. The American Heart Association (AHA) and Centers for Disease control (CDC) have defined risk groups as follows (4): Low risk: less than 1.0 (µg/mL or mg/L). Average risk: 1.0 – 3.0 (µg/mL or mg/L). High risk: Above 3.0 (µg/mL or mg/L). Studies conducted, in the United States and Europe, on subjects which did not have CVD at entry but may have had other risk factors (eg, hypercholesterolemia, hypertension, diabetes, tobacco use) have identified CRP as a strong, independent risk factor for CVD (5). CRP is classically measured using latex agglutination and nephelometric or turbidimetric methods. HS-CRP-CHECK-1 is a rapid quantitative screening test for the detection of CRP in serum, plasma or whole blood samples. The method relies on competitive binding of gold conjugate anti-CRP monoclonal antibodies with free CRP present in the sample and membrane coated CRP. Depending on CRP concentration in the sample, different lines will appear on the test, allowing the quantitative measurement of CRP in serum, plasma or whole blood samples, when used in combination with the EASY READER® or EASY READER+® rapid test readers.

Each kit contains

1. HS-CRP-CHECK-1 test devices: 10 20
2. Plastic tubes containing 2 mL diluent: 10 20
3. Instruction leaflet: 1 1
4. Controls (Optional): Positive control (ref. V340HS) and Negative control (ref. V341HS): a freeze-dried preparation of a non-infectious compound in diluted human serum, tested and found negative for anti- HIV, anti-HCV and HBs antigen, containing 0.05 % sodium azide is optionally available as a positive and negative control (1x 0.50 mL). The concentration range is indicated on the vial label.