HS-INSULIN-CHECK-1 High Sensitive Quantitative determination of Insulin in whole blood, plasma or serum sample

Human insulin is a peptide hormone produced in the pancreas in the Langerhans islets ß-cells as a proinsulin precursor which is then converted into C. peptide and insulin. Insulin is the main hormone involved in the control of glucose metabolism. ß-cells in the Langerhans islets release insulin in two phases. The first phase release is rapidly triggered in response to increased blood glucose levels. The second phase is a sustained, slow release of newly formed vesicles triggered independently of sugar. Insulin concentrations are severely reduced in insulin-dependent diabetes mellitus and some other conditions such as hypopituitarism. Insulin levels are raised in non-insulin-dependent diabetes mellitus, obesity, inulinoma and some endocrine dysfunctions such as Cushion’s syndrome and acromegaly . HS-INSULIN-CHECK-1 is a rapid quantitative assay for the detection of insulin in serum, plasma or whole blood samples. The method is based on an unique combination of monoclonal dye conjugate and polyclonal solid phase antibodies to identify insulin in the test samples with a high degree of specificity. As the test sample flows through the absorbent device, the labelled antibody-dye conjugate binds to the insulin forming an antibody- antigen complex. This complex binds to the anti-insulin antibody in the reaction zone (T) and produces a pink-rose coloured band. In the absence of insulin, there is no line in the reaction zone (T). The mixture continues flowing through the absorbent device past the reactive zone (T) and control zone (C). Unbound conjugate binds to the reagents in the control zone (C), producing a pink- rose coloured band, demonstrating that the reagents are functioning correctly.

Each kit contains

1. HS-INSULIN-CHECK-1 reaction devices: 10 20
2. Disposable plastic pipettes : 10 20
3. Diluent in a dropper bottle : 2.5mL 5mL
4. Instruction leaflet : 1 1