IgE-CHECK-1 Quantitative determination of immunoglobulin-E in whole blood, plasma or serum samples

Immunoglobulin E (IgE) is an immunoglobulin normally found in nanogram level in healthy individual (1, 2). In allergic patients, IgE is present both in the serum and also tightly bound to the surface of basophils and mast cells (3, 4). Screening the level of total IgE is of diagnostic use in case of asthma (to differentiate allergic from non allergic asthma), rhinitis and eczema (5). Other diseases in which elevated levels of serum IgE are found include parasitic infections, broncho-pulmonary aspergillosis and some dermatitis. The IgE-CHECK-1 is a rapid quantitative assay for the detection of IgE in whole blood, plasma or serum samples. The method employs a unique combination of monoclonal dye conjugate and polyclonal solid phase antibodies to identify IgE in the test samples with a high degree of sensitivity. As the test sample flows through the absorbent device, the antibody-dye conjugate binds to the IgE forming an antibody- antigen complex. This complex binds to the anti IgE antibody in the reaction zone (T) and produces a pink-rose colour band. In absence of IgE, the mixture continues flowing through the absorbent device past the reactive zone (T) and control zone (C). Unbound conjugate binds to the reagents in the control zone (C), producing a pink-rose colour band, demonstrating that the reagents are functioning correctly.

Each kit contains

1. IgE-CHECK-1 test devices 10 20
2. Disposable plastic pipettes 10 20
3. Diluent in a dropper bottle 2.5mL 5mL
4. Instruction leaflet 1 1
5. Controls (Optional): Positive control ref. V1890 and Negative control ref. V1891: a freeze-dried preparation of a non-infectious compound in diluted human serum, tested and found negative for anti-HIV, anti-HCV and HBs antigen, containing 0.05 % sodium azide is optionally available as a positive and negative control (1x 0.25 mL). The concentration range is indicated on the vial label.