MGL-CHECK-1 Quantitative determination of Myoglobin in whole blood, plasma or serum samples

Myoglobin is an intra cellular heme protein involved in the storage and transfer of oxygen to muscle tissues (1). In man, myoglobin is one of the major proteins found in the myocardium and therefore it is not surprising that injury to cardiac muscle results in the release of myoglobin into the bloodstream (2, 3). Normal levels in human serum are about 60-80 ng/mL with elevations as high as 1,500 ng/mL seen in patients with acute myocardial infarction (AMI). Consequently, myoglobin measurements are valuable indications in the early diagnosis of AMI as myoglobin has been reported to rise within 4 hours of the onset of symptoms (4, 5, 6). The MGL-CHECK-1 is a rapid quantitative assay for the detection of human myoglobin in serum, plasma or whole blood. The method employs a unique combination of monoclonal-dye conjugate and polyclonal solid phase antibodies to identify myoglobin in the test samples with a high degree of specificity. As the sample flows through the absorbent device, the labelled antibody-dye conjugate binds to the myoglobin forming an antibody antigen complex. This complex binds to the anti-myoglobin antibody in the reaction zone (T) and produces a pink-rose colour band. In the absence of myoglobin, there is no line in the reaction zone (T). The mixture continues flowing through the absorbent device past the reactive zone (T) and control zone (C). Unbound conjugate binds to the reagents in the control zone (C), producing a pink-rose colour band, demonstrating that the reagents are functioning correctly.

Each kit contains

1. MGL-CHECK-1 reaction devices 10 20
2. Disposable plastic pipettes 10 20
3. Diluent in a dropper bottle 2.5 mL 5 mL
4. Instructions leaflet 1 1
5. Controls (Optional): Positive control (ref. V2600) and Negative control (ref. V2601): a freeze-dried preparation of a non-infectious compound in diluted human serum, tested and found negative for anti-HIV, anti-HCV and HBs antigen, containing 0.05 % sodium azide and optionally available as a positive and negative control (1x 0.25 mL). The concentration range is indicated on the vial label.