PROLAC-CHECK-1 Quantitative determination of Prolactin in whole blood, plasma or serum samples

Human Prolactin (hPRL), which is secreted by the anterior lobe of the pituitary gland (1, 2), is essential for breast development and lactation in women. High levels can be detected after the eighth week of pregnancy and continue until term. After birth, prolactin levels return to normal within three weeks in the absence of breast feeding (3, 4). Normal females show prolactin levels only slightly higher than males (2, 3). Abnormally high levels of hPRL are associated with infertility in men and women, male impotence and primary hypothyroidism. The PROLAC-CHECK-1 is a rapid, quantitative assay for the detection of human prolactin in whole blood, serum and plasma samples. The method employs a unique combination of monoclonal-dye conjugate and polyclonal-solid phase antibodies to identify prolactin in the test samples selectively with a high degree of specificity. As the test sample flows through the absorbent device, the labelled antibody-dye conjugate binds to the prolactin forming an antibody-antigen complex. This complex binds to the anti- prolactin antibody in the reaction zone (T) and produces a rose-pink coloured band. In the absence of prolactin, there is no line in the reaction zone (T). The mixture continues flowing through the absorbent device, past the reaction zone (T) and control zone (C). Unbound conjugate binds to the reagents in the control zone (C), producing a rose-pink coloured band, demonstrating that the reagents are functioning correctly.

Each kit contains

1. PROLAC-CHECK-1 reaction devices 10 20
2. Disposable plastic pipettes 10 20
3. Diluent in a dropper bottle 2.5 mL 5 mL
4. Instructions leaflet 1 1
5. Controls (Optional): Positive control ref. V9000 and Negative control ref. V9001: a freeze-dried preparation of a non-infectious compound in diluted human serum, tested and found negative for anti-HIV, anti-HCV and HBs antigen, containing 0.05 % sodium azide is optionally available as a positive and negative control (1x 0.25 mL). The concentration range is indicated on the vial label.