TRANSFERRIN-CHECK-1 Quantitative determination of transferrin in faeces samples

Transferrin is a glycoprotein of around 80kDa which consists of a polypeptide strand with 2 N-glycosidically linked oligosaccharide chains (1). The main role of transferrin is iron transport from the absorption centers in the duodenum and white blood cell macrophages to all tissues (2). Therefore it is considered as an extremely sensitive indicator of iron deficiency (3). Further more, transferrin is present in the blood in relatively high concentration and is much more stable than haemoglobin especially in faeces samples. Therefore, transferrin has also been used recently to detect gastrointestinal bleedings occurring in the upper digestive tract cancers such as stomach and colorectal cancers (4). The TRANSFERRIN-CHECK-1 test is a quantitative immunochromatographic assay for the detection of transferrin in faeces. The method employs a unique combination of gold conjugate and solid phase monoclonal antibodies to selectively identify human transferrin with a high degree of sensitivity and specificity. After faeces sample collection in a specific collection device containing extraction solution, a few drops of the mixture are added to the sample well of the reaction device. As the liquid flows through the absorbent device, the labelled antibody-dye conjugate binds to the transferrin antigen (when present in the faeces sample) forming an antibody antigen complex. In the absence of transferrin, there is no line in the positive reaction zone (T). The reaction mixture continues flowing through the absorbent device, past the positive reaction zone (T) and control zone (C). Depending on the transferrin concentration level, different lines of different intensities will appear on the reading window allowing the quantitative measurement of transferrin when used in combination with the VEDALAB’s readers EASYREADER® or EASYREADER+®.

Each kit contains

1. TRANSFERRIN-CHECK-1 test devices 10 20
2. Faeces sample collection devices containing 2 mL of solution 10 20
3. Instructions leaflet 1 1