Vitamin D-CHECK-1 Quantitative determination of Vitamin D in whole blood, plasma or serum samples

Vitamin D is a lipid-soluble steroid hormone that exists in two primary forms: vitamin D2 (Ergocalciferol) and vitamin D3 (cholecalciferol). The Vitamin D3 is naturally produced in the skin through the exposure to ultraviolet light or found in certain food sources such as oily fish and variably fortified foods (milk, juices, soy, etc…) while Vitamin D2 is found in certain plants and mushrooms. The Vitamin D3 and D2 have a relatively high affinity for a α2- globulin which functions as a transport protein (Vitamin D binding protein) present in the blood. They must undergo a series of metabolic transformations in the liver and then in the kidney to its biologically active form 1-25-dihydroxyVitamin D (1, 2). The major circulating form of vitamin D is 25-OH-Vitamin D. For this reason, the bloodstream concentration of 25-OH-Vitamin D is considered as the best for Vitamin D status assessment and very well reflects Vitamin D3 and D2 inputs (3). The well-known action of this hormone is the regulation of calcium and phosphors homeostasis by enhancing intestinal absorption of calcium. This system plays an important role in bone metabolism. Abnormalities of this system can lead to bone deformation (commonly known as rickets) in infants and children while in adults, proximal muscle weakness, bone pain and increasing risk of osteoporosis may develop (4, 5). On the other hand, vitamin D also has non- phosphocalcic actions via an autocrine action that affects numerous physiological processes other than bone and mineral metabolism. Thus, vitamin D deficiency is also associated to non-musculoskeletal risks including increased risk of various chronic diseases, auto- immune diseases or cardiovascular risks etc… (6, 7). Deficiency in Vitamin D is therefore linked to various diseases and its prevalence is high in numerous countries, making it a worldwide problem (8, 9). Vitamin D-CHECK-1 test is a quantitative manual assay for the detection of 25-OH-Vitamin D in whole blood, plasma or serum samples. The method is based on a unique combination of monoclonal dye conjugate and solid phase antibodies to selectively detect 25-OH- Vitamin D with a high degree of sensitivity and specificity. Depending on the 25-OH-Vitamin D concentration in the sample, different lines intensities will appear on the test allowing its quantitative measurement when used in combination with VEDALAB’s EASYREADER+® instrument.

Each kit contains everything needed to perform 20 tests. - Aluminium pouches containing: 1 Vitamin D-CHECK-1 test device 1 desiccant bag - Disposable plastic pipettes in a protective plastic bag - Diluent in a dropper bottle - Leaflet 1 20 20 5 mL Material required but not provided: - Timer